Refereed Journal Articles:
57. Hagedorn M, Carter V, Zuchowicz N, Phillips M, Penfield C, Shamenek B, Vallen EA, Kleinhans FW, Peterson K, White M, Yancey PH. Trehalose is a chemical attractant in the establishment of coral symbiosis. PLoS One 10(1): e0117087 (2015).
56. Kleinhans FW and Mazur P. Physical parameters, modeling, and methodological details in using IR laser pulses to warm frozen or vitrified cells ultra-rapidly. Cryobiology 70, 195-203 (2015).
55. Jin B, Kleinhans FW, Mazur P. Survivals of mouse oocytes approach 100% after vitrification in 3-fold diluted media and ultra-rapid warming by an IR laser pulse. Cryobiology 68, 419-430 (2014).
54. Peckys DB, Kleinhans FW, Mazur P.
Rectification of the water permeability in COS-7 cells at 22, 10 and 0C.
PLoS One, 6(8):e23643, (2011).
53. Hagedorn M, Carter VL, Leong JC, Kleinhans FW. Physiology and cryosensitivity of coral endosymbiotic algae (Symbiodinium). Cryobiology 60, 147-158 (2010).
52. Hagedorn M, Carter VL, Ly S, Andrell RM, Yancey PH, Leong JA, Kleinhans FW. Analysis of internal osmolality in developing coral larvae, Fungia scutaria. Physiol Biochem Zool. 83, 157-66 (2010).
51. Kleinhans FW, Seki S, Mazur P. Simple, inexpensive attainment and measurement of very high cooling and warming rates. Cryobiology 61, 231-233 (2010).
50. Kleinhans FW, Mazur P. Determination of the water permeability (Lp) of mouse oocytes at -25 degrees C and its activation energy at subzero temperatures. Cryobiology 58, 215-224 (2009).
49. Seki S, Kleinhans FW, Mazur P. Intracellular ice formation in yeast cells vs. cooling rate: predictions from modeling vs. experimental observations by differential scanning calorimetry. Cryobiology 58, 157-165 (2009).
48. Hagedorn M, Ricker J, McCarthy M, Meyers SA, Tiersch TR, Varga ZM, Kleinhans FW. Biophysics of zebrafish (Danio rerio) sperm. Cryobiology 58, 12-19 (2009).
47. Peter Mazur and F.W.
Kleinhans. Relationship between intracellular ice formation in oocytes of
the mouse and Xenopus and the physical state of the external medium-A revisit.
Cryobiology 56, 22-27 (2008).
46. Mazur P, Pinn IL, Kleinhans FW. Intracellular ice formation in mouse oocytes subjected to interrupted rapid cooling. Cryobiology 55, 158-166 (2007).
45. Mazur P, Pinn IL, Kleinhans FW. The temperature of intracellular ice formation in mouse oocytes vs. the unfrozen fraction at that temperature. Cryobiology 54, 223-233 (2007).
44. Kleinhans FW and Mazur P. Comparison of actual vs. synthesized ternary phase diagrams for solutes of cryobiological interest. Cryobiology 54, 212-222 (2007).
43. Yamaji Y, Valdez DM Jr, Seki S, Yazawa K, Urakawa C, Jin B, Kasai M, Kleinhans FW, Edashige K. Cryoprotectant permeability of aquaporin-3 expressed in Xenopus oocytes. Cryobiology 53, 258-267 (2006).
42. Edashige K, Tanaka M, Ichimaru N, Ota S, Yazawa K, Higashino Y, Sakamoto M, Yamaji Y, Kuwano T, Valdez DM Jr, Kleinhans FW, Kasai M. Channel-Dependent Permeation of Water and Glycerol in Mouse Morulae. Biol.Reprod. 74, 625-632 (2006).
41. JF Guenther, Shinsuke Sekia, FW Kleinhans, Keisuke Edashige, DM Roberts, and Peter Mazur. Extra- and intra-cellular ice formation in stage I and II Xenopus laevis oocytes. Cryobiology 52, 401-416 (2006).
40. Kleinhans FW, Guenther JF, Roberts DM, Mazur P. Analysis of intracellular ice nucleation in Xenopus oocytes by differential scanning calorimetry. Cryobiology 52, 128-138 (2006).
39. Hagedorn M, Pan R, Cox EF, Hollingsworth L, Krupp D, Lewis TD, Leong JC, Mazur P, Rall WF, Macfarlane DR, Fahy G, Kleinhans FW. Coral larvae conservation: Physiology and Reproduction. Cryobiology 52, 33-47 (2006).
38. Mazur P, Pinn IL, Seki S, Kleinhans FW, Edashige K. Effects of hold time after extracellular ice formation on intracellular freezing of mouse oocytes. Cryobiology 51, 235-9 (2005).
37. Mazur P, Seki S, Pinn IL, Kleinhans FW, Edashige K. Extra- and intracellular ice formation in mouse oocytes. Cryobiology 51, 29-53 (2005).
36. M Hagedorn, A Peterson, P Mazur, and FW Kleinhans. High ice nucleation temperatures of zebrafish embryos: slow-freezing is not an option. Cryobiology 49, 181-189 (2004)
35. SL Adams, FW Kleinhans, PV Mladenov, and PA Hessian. Membrane permeability characteristics and osmotic tolerance limits of sea urchin (Evechinus chloroticus) eggs. Cryobiology 47, 1-13 (2003)
34. K Edashige, Y Yamaji, FW Kleinhans, and Magosaburo Kasai. Artificial Expression of Aquaporin-3 Improves the Survival of Mouse Oocytes after Cryopreservation. Biology of Reproduction 68, 87-94 (2003)
33. M Hagedorn, SL Lance, D Fonseca, FW Kleinhans, D Artimov, R Fleischer, A.T.M. S. Hoque, M Hamilton, and B Pukazhenthi. Altering fish embryos with aquaporin-3: an essential step toward successful cryopreservation. Biology of Reproduction 67, 961-966 (2002)
32. MR Curry, FW Kleinhans, and PF Watson. Measurement of the water permeability of the membranes of boar, ram, and rabbit spermatozoa using concentration dependent self-quenching of an entrapped fluorophore. Cryobiology 41, 167-173 (2000)
31. M. Janik, F.W. Kleinhans, and M. Hagedorn. Overcoming a permeability barrier by microinjecting cryoprotectants into zebrafish embryos (Brachydanio rerio). Cryobiology 41, 25-34 (2000)
30. F.W. Kleinhans. Invited Review: Membrane permeability modeling: Kedem-Katchalsky vs a two parameter formalism. Cryobiology 37, 271-289 (1998)
29. M. Hagedorn, F.W. Kleinhans, D. Artemov, and U. Pilatus. Characterization of a major permeability barrier in the zebrafish embryo. Bio of Reprod. 59, 1240-1250 (1998)
28. M. Hagedorn, F.W. Kleinhans, D.E. Wildt, and W.F. Rall. Chill sensitivity and cryoprotectant permeability of dechorionated zebrafish embryos, Brachydanio rerio. Cryobiology 34, 251-263 (1997).
27. M. Hagedorn, F.W. Kleinhans, R. Freitas, J. Liu, E. Hsu, D.E. Wildt, and W.F. Rall. Water distribution and permeability of zebrafish embryos, Brachydanio rerio. Journal of Experimental Zoology 278, 356-367 (1997).
26. F.W. Kleinhans and M. Hagedorn. Appendix (to above): Measurement of intracellular water volume in multicompartmental systems such as the zebrafish embryo. Journal of Experimental Zoology 278, 368-371 (1997).
25. A.N. Zaplatin, Kent A. Baker, and F.W. Kleinhans. Effectiveness and toxicity of several DTPA broadening agents for biological ESR spectroscopy. Journal of Magnetic Resonance B 110, 249-254 (1996).
24. J.A. Gilmore, L.E. McGann, J. Liu, D.Y. Gao, A.T. Peter, F.W. Kleinhans, and J.K. Critser. The effect of cryoprotectant solutes on water permeability of human spermatozoa. Biology of Reproduction 53, 985-995 (1995).
23. Jun Tao, Junying Du, F.W. Kleinhans, E.S. Critser, P. Mazur, and J.K. Critser. The effect of collection temperature, cooling rate and warming rate on chilling injury and cryopreservation of mouse spermatozoa. Journal of Reproduction and Fertility 104, 231-236 (1995).
22. D.Y. Gao, J. Liu, L.E. McGann, P.F. Watson, F.W. Kleinhans, P. Mazur, E.S. Critser, and J.K. Critser. Prevention of osmotic injury to human spermatozoa during addition and removal of glycerol. Human Reproduction 10, 1109-1122 (1995).
21. Junying Du, Jun Tao, F.W. Kleinhans, A.T. Peter, and J.K. Critser. Determination of boar spermatozoa water volume and osmotic response. Theriogenology 42, 1183-1191 (1994).
20. Junying Du, F.W. Kleinhans, Peter Mazur, J.K. Critser. Human spermatozoa glycerol permeability and activation energy determined by electron paramagnetic resonance. Biochimica Biophysica Acta 1194, 1-11 (1994).
19. Junying Du, Jun Tao, F.W. Kleinhans, P. Mazur, and J.K. Critser. Water volume and osmotic behavior of mouse spermatozoa determined by electron paramagnetic resonance. Journal of Reproduction and Fertility 101, 37-42 (1994).
18. Junying Du, F.W. Kleinhans, P. Mazur and J.K. Critser, Osmotic behavior of human spermatozoa studied by EPR. Cryo-Letters 14, 285-294 (1993).
17. D.Y. Gao, E. Ashworth, P.F. Watson, F.W. Kleinhans, P. Mazur and J.K. Critser, Hyperosmotic tolerance of human spermatozoa: Separate effects of glycerol, sodium chloride and sucrose on spermolysis. Biology of Reproduction 49, 112-123 (1993).
16. E.E. Noiles, P. Mazur, P.F. Watson, F.W. Kleinhans and J.K. Critser. Determination of Water Permeability Coefficient for Human Spermatozoa and its Activation Energy. Biology of Reproduction 48, 99-109 (1993).
15. D.Y. Gao, P. Mazur, P.F. Watson, F.W. Kleinhans, P.F. Watson, E.E. Noiles and J.K. Critser, Glycerol Permeability of Human Spermatozoa and its Activation Energy. Cryobiology 29, 657-667 (1992).
14. F.W. Kleinhans, V.S. Travis, Junying Du, P.M. Villines, K.E. Colvin and J.K. Critser. Measurement of Human Sperm Intracellular Water Volume by ESR. Journal of Andrology 13, 498-506, (1992).
13. N.D. Lees, F.W. Kleinhans, M.C. Broughton, D.E. Pennington, V.A. Ricker and M. Bard, Membrane Fluidity Alterations in a Cytochrome P-450 Deficient Mutant of Candida albicans, Steroids 53, 567-578 (1989).
12. J.J. Gibboney, R.A. Haak, F.W. Kleinhans, and Z. Brahmi, Electron Spin Resonance Spectroscopy Does Not Reveal Hydroxyl Radical Production in Activated Natural Killer Lymphocytes, J. of Leukocyte Biology 44, 545-550 (1988).
11. F.W. Kleinhans and S.T. Barefoot, Spin Trap Determination of Free Radical Burst Kinetics in Stimulated Neutrophils, J. Biol. Chem. 262, 12,452-7 (1987).
10. F.W. Kleinhans, A Noise Reduction Scheme for Computer Controlled EPR Spectrometers, J. Mag. Res. 65, 146-148 (1985).
9. R.L. Berkow, S.J. Weisman, D. Tzeng, R.A. Haak, F.W. Kleinhans, S.T. Barefoot and R.L. Baehner, The Comparative Response of Human PMN Obtained by Counterflow Centrifugal Elutriation and Ficoll-Hypaque Density Centrifugation, Part II, Journal of Lab. and Clin. Med. 104, 698-710 (1984).
8. F.W. Kleinhans, S.C. Kline, W.M. Dugan, J.G. Williams, Comparison of Electron Paramagnetic Resonance and Atomic Absorption Serum Copper Measurements in Human Normals and Cancer Patients, Cancer Research 43, 3447-3450 (1983).
7. Dean A. Hawley, F.W. Kleinhans, and James L. Biesecker, Determination of Alternate Pathway Complement Kinetics by Electron Spin Resonance Spectroscopy, American Journal of Clinical Pathology 79, 673-677 (1983).
6. W.J. Newhall, F.W. Kleinhans, R.S. Rosenthal, W.D. Sawyer and R.A. Haak, Neisseria gonorrhoeae Membrane Microenvironment Studies by Spin-Label Electron Spin Resonance: Comparison of Colony Types, J. Bact. 139, 98-106 (1979).
5. N.D. Lees, M. Bard, M.D. Kemple, R.A. Haak, and F.W. Kleinhans, ESR Determination of Membrane Order Parameters in Yeast Sterol Mutants, Biochim. Biophys. Acta 553, 469-475 (1979).
4. F.W. Kleinhans, M. Bard, N.D. Lees, and R.A. Haak, ESR Determination of Membrane Permeability in a Yeast Sterol Mutant, Chemistry and Physics of Lipids 23, 143-154 (1979).
3. M. Bard, N.D. Lees, L.S. Burrows, and F.W. Kleinhans, Differences in Crystal Violet Uptake and Cation Induced Death among Yeast Sterol Mutants, Journal of Bacteriology 135, 1146-1148 (1978).
2. R.A. Haak, F.W. Kleinhans, and S. Ochs, The Viscosity of Mammalian Nerve Axoplasm Measured by Electron Spin Resonance, Journal of Physiology 263, 115-1237 (1976).
1. F.W. Kleinhans, J.P. Long and P.E. Wigen, Antiferromagnetic Resonance in Mg-Mn Alloys, Phys. Rev. B 11, 2638-2641 (1975).
Invited Presentations:
5. FW Kleinhans, Basic Cryobiology and Biophysics of Cells at Cold Temperatures. Symposium on Cryobiology of Fish Sperm Cryopreservation, West Coast Zebrafish Meeting, University of Oregon, Eugene, OR. Sept 9-11 (2005).
4. FW Kleinhans, Biophysical and Osmotic Properties of the Coral, Fungia. Coral Workshop, Uni of Hawaii Marine Biology Institute, July 1-10 (2004).
3. F.W. Kleinhans, Membrane permeability modeling: issues and pitfalls. Cryo' 99, Marseille, France, July 12-15 (1999).
2. F.W. Kleinhans, What Next? Are we cryobiologists, membrane biophysicists, or molecular biologists? Cryo' 99, Marseille, France, July 12-15 (1999).
1. F.W. Kleinhans. Membranes, permeability, and osmotic relations. International (NIH) Workshop on Comparative Gamete and Embryo Cryopreservation. Yerkes Regional Primate Research Ctr, Emory University, Atlanta, Georgia. March 19-20 (1998).
1. M. Hagedorn and F.W. Kleinhans. Cryopreservation of Fish Embryos: Is it within the foreseeable future for aquaculture in Cryopreservation of Gametes and Embryos of Aquatic Species. Eds. T. Tiersch and P. Mazik, World Aquaculture Society, Seattle (1999)
6. M. Hagedorn, E. Hsu, F.W. Kleinhans, and D.E. Wildt. Approaches for studying the permeability of fish embryos: Toward successful cryopreservation. Cryobiology 34, 335-347 (1997).
5. D.Y. Gao, J. Liu, F.W. Kleinhans, A. Ungan, and J.K. Critser. On a lumped heat transfer model of freezing process of cryopreservation media. Advances in Cryogenic Engineering 41, 29-36 (1996), Edited by P. Kittel, Plenum Press, NY.
4. E.E. Noiles, N.A. Ruffing, F.W. Kleinhans, L.A. Mark, L. Horstmann, P.F. Watson, P. Mazur and J.K. Critser, Critical Tonicity Determination of Sperm using Fluorescent Staining and Flow Cytometry, Reproduction in Domestic Animals, Suppl. 1, Boar Semen Preservation II (Proceedings of the Second International Conference on Boar Semen Preservation, 1990), 359-364 (1991).
3. J. Du, F.W. Kleinhans, V.J. Spitzer, L. Horstmann, P. Mazur and J.K. Critser, ESR-Determined Osmotic Behavior of Bull Spermatozoa, Reproduction in Domestic Animals, Suppl. 1, Boar Semen Preservation II (Proceedings of the Second International Conference on Boar Semen Preservation, 1990), 105-108 (1991).
2. R.A. Haak, W.J. Newhall, F.W. Kleinhans, W.D. Sawyer, R.S. Rosenthal, L.F. Guyman, and P.F. Sparling, Altered Membrane Fluidity of Drug-Susceptible and -Resistant Neisseria gonorrhoeae, in Immunobiology of Neisseria gonorrhoeae, Ed.: Geo. F. Brooks, et al., American Society for Microbiology, Washington, D.C., 142-144 (1978).
1. F.W. Kleinhans and P.E. Wigen, Electron Paramagnetic Resonance in Mg-Mn, AIP Conf. Proc. 5, Magnetism and Magnetic Materials, 1204-1208 (1971).
3. F Kleinhans, Show Me the Dog, Science 311, 1858-1859 (2006).
2. F.W. Kleinhans, Galaxy in Virgo: Aesthetically Uninteresting? Science 271, 1655 (1996).
1. F.W. Kleinhans, Comet Hyakutake Photo, Indianapolis Star, April 3 (1996).
88. FW Kleinhans, Bo Jin, Estefania Paredes, and Peter Mazur. Ultra rapid warming of cryo samples using an IR laser pulse. Society for Cryobiology Annual Meeting, July 2013, Washington DC. Cryobiology 67, 424 (2013).
87. Bo Jin, FW Kleinhans, and Peter Mazur. Survival of mouse oocytes after cooling in lower cryoprotectant concentrations by ultra rapid warming using an IR laser pulse. Society for Cryobiology Annual Meeting, July 2013, Washington DC. Cryobiology 67, 408 (2013).
86. FW Kleinhans, Shinsuke Seki, Peter Mazur. Simple, inexpensive measurement of very rapid cooling and warming rates. Society for Cryobiology Annual Meeting, July 19-23, 2009, Sapporo Japan. Cryobiology 59, 381 (2009).
85. Shinsuke
Seki, FW Kleinhans, and Peter Mazur. Detection of intracellular ice
formation in cells of the yeast Saccharomyces cerevisiae by differential
scanning calorimetry. Society for Cryobiology Annual Meeting, July 20-23,
2008, Charlotte, NC. Cryobiology 57, 325 (2008).
84. Mary Hagedorn, Josette Ricker, Stuart Meyers, Terrence Tiersch, and Frederick Kleinhans. Biophysics of Zebrafish Danio rerio Sperm. 41st SSR (Society for the Study of Reproduction) Annual Meeting, May 27-30, 2008, Kailua-Kona, Hawaii. (2008).
83. Peter Mazur, Irina L.
Pinn and F.W. Kleinhans. Intracellular ice formation in mouse oocytes
subjected to interrupted rapid cooling. Society for Cryobiology, Banff, CA, July
28-Aug 1, 2007. Cryobiology, 55, 327 (2007).
82. Peter Mazur, Irina L. Pinn and F.W. Kleinhans. The temperature of intracellular ice formation in mouse oocytes vs. the unfrozen fraction at that temperature. Society for Cryobiology, Banff, CA, July 28-Aug 1, 2007. Cryobiology, 55, 327-328 (2007).
81. F.W. Kleinhans and Peter Mazur. Sub zero water permeability, Lp, of mouse oocytes. Society for Cryobiology, Banff, CA, July 28-Aug 1, 2007. Cryobiology 55, 341 (2007).
80. M Hagedorn, R Steyn, VL Carter, RP Lang, DA Krupp, and FW Kleinhans. Coral conservation through cryopreservation. 2006 Ocean Sciences Meeting, Honolulu, HI, Feb 20-24, 2006. EOS Trans. AGU 87(36), Ocean Sci. Meet. Suppl, OS26G-23 (2006).
79. M. Hagedorn, R Pan, EF Cox, L Hollingsworth, D Krupp, TD Lewis, JC Leong, P Mazur, WF Rall, DR MacFarlane, G Fahy, and FW Kleinhans. Coral conservation and cryopreservation. 42nd Annual Meeting – Society for Cryobiology, Minneapolis, MN, July 24-27, 2005. Cryobiology 51, 352 (2005).
78. F.W. Kleinhans, J.F. Guenther, S. Seki, K. Edashige, D.M. Roberts, P. Mazur. Water Pf of Xenopus (AQP1 ±) stage I and II oocytes. 42nd Annual Meeting – Society for Cryobiology, Minneapolis, MN, July 24-27, 2005. Cryobiology 51, 389-390 (2005).
77. F.W. Kleinhans, J.F. Guenther, D.M. Roberts, P. Mazur. Analysis of intracellular ice nucleation in early stage Xenopus oocytes by differential scanning calorimetry. 42nd Annual Meeting – Society for Cryobiology, Minneapolis, MN, July 24-27, 2005. Cryobiology 51, 389 (2005).
76. M. Hagedorn, R. Pan, E.F. Cox, L. Hollingsworth*, D. Krupp, T.D. Lewis, J.C. Leong, P. Mazur, W.F. Rall, D.R. MacFarlane, G. Fahy, F.W. Kleinhans. Coral conservation and cryopreservation. 42nd Annual Meeting – Society for Cryobiology, Minneapolis, MN, July 24-27, 2005. Cryobiology 51, 352 (2005).
75. FW Kleinhans, P Mazur, J Gunther, S Seki, K Edashige, and D Roberts. Water Pf of Xenopus (AQP1 +/-) Stage I & II Ooctyes. Uni of Tenn, Knoxville, BCMB Biology Retreat, May 7, 2004.
74. P Mazur, FW Kleinhans, S Seki, J Guenther, K Edashige, and D Roberts. Factors affecting intracellular ice nucleation in mouse and Xenopus oocytes. BCMB Biology Retreat, Uni Tenn Knoxville, May 7, 2004.
73. M Hagedorn, A Peterson, P Mazur, and FW Kleinhans, High Ice Nucleation Temperature of Zebrafish Embryos. CryoBioMol 2003, Sept 14-18, 2003, Coimbra, Portugal. Cryobiology 47 p290 (2003).
72. K Edashige, N Ichimaru, Y Higashino, M Kasai, and FW Kleinhans, Water permeation in mouse morulae relies on water channels. Annual Meeting 2002, Society for Cryobiology, July 28 - 31, Breckenridge, CO. Cryobiology 45, 271 (2002).
71. SL Adams, FW Kleinhans, PA Hessian, and PV Mladenov, Membrane permeability of eggs from the Sea Urchin, Evechinus chloroticus. Annual Meeting 2002, Society for Cryobiology, July 28 - 31, Breckenridge, CO. Cryobiology 45, 269-270 (2002).
70. M Hagedorn and FW Kleinhans, Asymmetry in membrane permeability in zebrafish embryos. Annual Meeting 2001, Society for Cryobiology, 7-29 to 8-1, University of Edinburgh, Scotland. Cryobiology 43, 339 (2001).
69. K Edashige, Y Yamaji, M Kasai, and FW Kleinhans. Cryoprotectant permeability of aquaporin 3 expressed in xenopus oocytes. Annual Meeting of the Society for Cryobiology, Boston Mass, July 30-Aug 1, 2000. Cryobiology 41, 384 (2000).
68. M Janik, FW Kleinhans, and M Hagedorn. Microinjection of cryoprotectants into the yolk of zebrafish embryos (Brachydanio rerio). SSR Meeting, July 15-18, Madison, WI. Biology of Reproduction 62 (Suppl 1), 146 (2000)
67. F.W. Kleinhans, KC Cole, V Wangia, A Laughinghouse, and Peter Mazur. The use of electroporation to permeabilize the vitelline membrane of Anopheles gambiae to water and cryoprotectant. Entomological Society of America Annual Meeting, Dec 12-16, Atlanta, GA (1999).
66. F.W. Kleinhans. Membrane permeability modeling: Issues and Pitfalls. 36th Annual Meeting of the Society for Cryobiology, July 12-15, Marseille, France. Cryobiology 39, 296-297 (1999).
65. I.I. Katkov, F.W. Kleinhans, and Peter Mazur. Permeabilization of malaria mosquito eggs by electroporation. 35th Annual Meeting of the Society for Cryobiology, July 11-16, Pittsburgh, PA. Cryobiology 37, 393 (1998).
64. F.W. Kleinhans. Membrane permeability modeling: Use and misuse of the Kedem-Katchalsky formalism. 35th Annual Meeting of the Society for Cryobiology, July 11-16, Pittsburgh, PA. Cryobiology 37, 406 (1998).
63. F.W. Kleinhans and Mary Hagedorn. Zebrafish embryo modeling: Are two compartments better than one? 35th Annual Meeting of the Society for Cryobiology, July 11-16, Pittsburgh, PA. Cryobiology 37, 423 (1998).
62. M. Hagedorn, D. Artemov, U. Pilatus, and F. W. Kleinhans. Characterization and consequences of permeability barriers in zebrafish embryos. 35th Annual Meeting of the Society for Cryobiology, July 11-16, Pittsburgh, PA. Cryobiology 37, 424 (1998).
61. M. Hagedorn, F.W. Kleinhans, E. Hsu, U. Pilatus, D. Wildt, S. Blackband, and W. Rall. Cryopreservation of fish embryos. International Symposium of the biology of tropical fishes. Manaus, Brazil. Oct 6-9 (1997).
60. M. Hagedorn, E. Reed, U. Pilatus, D. Artemov, F.W. Kleinhans, and S.J. Blackband. Cryoprotectant permeability of dechorionated zebrafish embryos using MR spectroscopy. 8th Annual Noahs Days Conference, April 27-29, Conservation and Research Ctr, Smithsonian Institution, National Zoological Park, Front Royal, VA (1997).
59. M. Hagedorn, F.W. Kleinhans, E. Hsu, U. Pilatus, D.E. Wildt, S.J. Blackband, and W. Rall. Permeability studies of dechorionated zebrafish embryos: another difficult model. NIH Workshop on transgenic mouse sperm cryopreservation. May 19-20, Washington, DC (1997).
58. M. Hagedorn, E. Reed, U. Pilatus, D. Artemov, F. W. Kleinhans, and S.J. Blackband. Cryoprotectant permeability of dechorionated zebrafish embryos using MR spectroscopy. Eighth Annual NOAHS DAYS, Conservation and Research Center, National Zoological Park, Smithsonian Institution. Front Royal, VA. April 27-29 (1997).
57. F.W. Kleinhans and M. Hagedorn. Modeling of permeability parameters in dechorionated zebrafish embryos. The 33rd Annual Meeting of the Society for Cryobiology, August 17-21, 1996, Indianapolis, IN. Cryobiology 33, 647 (1996).
56. M. Hagedorn, F.W. Kleinhans, D.E. Wildt, and W. Rall. Water permeability studies in dechorionated zebrafish embryos. The 33rd Annual Meeting of the Society for Cryobiology, August 17-21, 1996, Indianapolis, IN. Cryobiology 33, 646-647 (1996).
55. J. Liu, D.Y. Gao, C.J. Sun, F.W. Kleinhans, and J.K. Critser. A model of cell water loss kinetics in glycerol media for constant and real cooling rates. Annual Cryobiology Meeting, 7/6/95-7/11/95, Madison Wisconsin. Cryobiology 32, 588 (1995).
54. R.T. Pfaff, J. Liu, A.T. Peter, F.W. Kleinhans, and J.K. Critser. Permeability characteristics of early mouse embryos. Annual Cryobiology Meeting, 7/6/95-7/11/95, Madison Wisconsin. Cryobiology 32, 568 (1995).
53. K.A. Baker, F.W. Kleinhans, and J.K. Critser. The diffusional water permeability (Pf) of human spermatozoa. Annual Cryobiology Meeting, 7/6/95-7/11/95, Madison Wisconsin. Cryobiology 32, 564 (1995).
52. C.E. Minks, A.T. Peter, P. Mazur, F.W. Kleinhans, and J.K. Critser. Osmotic behavior of mouse spermatozoa. American Society of Andrology Meeting, 3/31/95 - 4/4/95 in Raleigh NC. Journal of Andrology 16 (Supplement), P-33 (1995).
51. A.N. Zaplatin, Kent A. Baker, J.K. Critser, and F.W. Kleinhans. GD-DTPA as a potential broadening agent for ESR spectroscopy. 26th Southeastern Magnetic Resonance Conference, University of North Carolina at Chapel Hill, NC. Oct 23-25 (1994).
50. F. W. Kleinhans, Junying Du, Jun Tao, L. Horstman, V. S. Travis, K. E. Colvin, Peter Mazur and J. K. Critser. EPR studies of mammalian sperm, Workshop on In Vivo EPR and EPR of Viable Biological Systems. Dartmouth Medical Sch, NH, Oct 17-22 (1993).
49. J. Du, J. Tao, F.W. Kleinhans, P. Mazur, and J.K. Critser, The osmotic response of mouse spermatozoa measured by EPR. Cryobiology Meeting, Atlanta, Georgia, July 19 -23, 1993. Cryobiology 30, 633 (1993).
48. D. Gao, L. Mark, L. McGann, Jun Liu, F. W. Kleinhans, P. Mazur, and J. K. Critser. Prevention of osmotic injury of human spermatozoa during addition and removal of cryoprotective agent (CPA): Modeling and optimization. Cryobiology Meeting, Atlanta, Georgia, July 19-23 1993. Cryobiology 30, 649 (1993).
47. J. Du, J. Tao, F. W. Kleinhans, P. Mazur, and J. K. Critser. Mouse spermatozoa water volume determined by EPR. American Society of Andrology, Tampa, Fl, April 16-19, 1993. Journal of Andrology 14, 62 (1993).
46. E. E. Noiles, P. Mazur, F. W. Benker, F. W. Kleinhans, R. P. Amann and J. K. Critser, Critical Osmolality, Water and Glycerol Permeability Coefficient Determination of Equine Spermatozoa, Society for the Study of Reproduction, Raleigh, NC, July 13-15, 1992. Biology of Reproduction 46 (Suppl 1), 95 (1992).
45. E. E. Noiles, P. Mazur, F. W. Kleinhans and J. K. Critser, Water Permeability of Human Sperm in the Presence of Intracellular Glycerol, Cryobiology Meeting, Cornell University, Ithaca, NY, June 14-19, 1992. Cryobiology 29, 736 (1992).
44. J. Du, F. W. Kleinhans, P. Mazur and J. K. Critser, Osmotic Behavior of Human Spermatozoa Studied by EPR, Cryobiology Meeting, Cornell University, Ithaca, NY, June 14-19, 1992. Cryobiology 29, 736 (1992).
43. E. E. Noiles, P. Mazur, H. D. Boldt, F. W. Kleinhans, and J. K. Critser, Hydraulic Conductivity (Lp) and Its Activation Energy (Ea) in Human Sperm, J. Andrology Jan/Feb Suppl., 30 (1992).
42. D. Y. Gao, E. E. Noiles, F. W. Kleinhans, P. Mazur and J. K. Critser, Glycerol Permeability and its Activation Energy of Human Spermatozoa. J. Andrology Jan/Feb Suppl., 29 (1992).
41. J. Du, F. W. Kleinhans, P. Mazur and J. K. Critser, Permeability of the Human Spermatozoa to Glycerol Determined by EPR. Bulletin of the American Physical Society 37, 625 (1992).
40. J. K. Critser, F. W. Kleinhans, L. Horstman, P. Mazur and J. K. Critser, EPR Determination of Human and Bovine Spermatozoa Glycerol Permeability, Twenty-Third Southeastern Magnetic Resonance Conference, Atlanta, GA, Oct. 3-5, 1991.
39. J. K. Critser, F. W. Kleinhans, and P. Mazur, Cryopreservation of Human Sperm. Society for Cryobiology, Leuven, Belgium, July 7-12, 1991.
38. E. E. Noiles, N. A. Ruffing, F. W. Kleinhans, L. A. Mark, L. Horstman, P. F. Watson, P. Mazur and J. K. Critser, Critical Tonicity Determination of Semen Using Dual Fluorescent Staining and Flow Cytometry, Proc. Second International Conference on Boar Semen Preservation, Beltsville, MD, Aug. 19-22, 1990.
37. F. W. Kleinhans, J. Du, V. J. Spitzer, L. Horstman, P. Mazur and J. K. Critser, ESR-Determined Osmotic Behavior of Bull Spermatozoa, Proc. Second International Conference on Boar Semen Preservation, Beltsville, MD, Aug. 19-22, 1990.
36. R. S. Kincade, K. E. Colvin, F. W. Kleinhans, E. S. Critser, P. Mazur, J. K. Critser, The Effects of Cooling Rate and Repeated Freezing on Human Sperm Cryosurvival, J. Andrology 10, 48P (1989), American Society of Andrology, New Orleans, LA, April 1989.
35. F. W. Kleinhans, V. J. Spitzer, P. Villines, K. E. Colvin and J. K. Critser, ESR Determination of Human Sperm Cell Volume, J. Andrology 10, 30P (1989), American Society of Andrology, New Orleans, LA, April 1989.
34. V. J. Spitzer, F. W. Kleinhans, P. Villines, K. E. Colvin and J. K. Critser, ESR Determination of Cell Volumes, Indiana Academy of Science, Notre Dame, Nov. 10-11, 1988.
33. K. E. Colvin, F. W. Kleinhans, E. S. Critser and J. K. Critser, The Effect of Chromium Oxalate and Tempone on Human Sperm, Biol. Reprod. 38 suppl 1, p. 89 (1988).
32. F. W. Kleinhans, D. D. Nguyen and S. T. Barefoot, Effect of Pentoxifylline on ESR Measured RBC Fluidity, Indiana Academy of Science, Terre Haute, IN, Nov. 1987.
31. B. D. Bhagwandin, F. W. Kleinhans, and S. T. Barefoot, Spin Trap Assay of Hydroxyl and Superoxide Radicals from Stimulated Neutrophils, 18th Southeast Magnetic Resonance Conference, Vanderbilt University, Nashville, TN, Oct. 8-10, 1986.
30. B. D. Bhagwandin, F. W. Kleinhans, S. T. Barefoot, Kinetics of Hydroxyl Radical (OH•) Production from Stimulated Neutrophils, Federation Proceedings 45, 308 (1986), Federation American Society of Experimental Biology, St. Louis, MO, April 13-18, 1986.
29. B. Bhagwandin, S. T. Barefoot and F. W. Kleinhans, Lag Time of OH Production by Zymosan Stimulated Neutrophils, Proc. Indiana Acad. Sci. 95, 121 (1986), Indiana Academy of Science, Bloomington, IN, Nov. 1985.
28. M. Hill, F. W. Kleinhans, and S. T. Barefoot, Determination of OH Production by Stimulated Neutrophils using ESR Spectroscopy, Proc. Indiana Acad. Sci. 95, 121 (1986), Indiana Academy of Science, Bloomington, IN, Nov. 1985.
27. F. W. Kleinhans, Rapid Acquisition of Spin Label Signals, 17th Southeastern Magnetic Resonance Conference, Tuscaloosa, AL, Oct. 2-4, 1985.
26. R. A. Haak, S. T. Barefoot and F. W. Kleinhans, Disorders in Leukocyte Functions as Determined by EPR and Spin Trapping, 8th International Electron Paramagnetic Resonance Symposium, Denver, CO, July 1985.
25. F. W. Kleinhans, S. T. Barefoot and R. A. Haak, Neutrophil Metabolic Burst Profile via ESR, 8th International Electron Paramagnetic Resonance Symposium, Abstract No. 205, Denver, CO, July 1985.
24. S. T. Barefoot, F. W. Kleinhans, P. D. Toth, Platelet Activating Factor (PAF) Activation of Neutrophils is Selectively Blocked by a PAF Analog, Federation Proc. 44, 1041 (1985), Anaheim, CA, April 1985.
23. F. W. Kleinhans and S. T. Barefoot, Kinetics of the Neutrophil Metabolic Burst via ESR, Biophysical J. 47, 405a (1985), Baltimore, MD, Feb. 1985.
22. M. Hill, P. Gallager, F. W. Kleinhans, EPR Measurement of Lyposomal Leakage, Proc. Indiana Academy of Science 94, 170-171 (1985), Indianapolis, IN, Oct. 1984.
21. S. T. Barefoot, P. D. Toth, F. W. Kleinhans, and S. A. Hamburger, Hydroxyl Radical Production by Neutrophils: Effect of Endotoxic Shock, Circulatory Shock 13, 87 (1984), Toronto, Canada, June 1984.
20. S. T. Barefoot and F. W. Kleinhans, Phorbol Myristate Acetate Reduces Calcium Requirements for Neutrophil Stimulation, Federation Proc. 43, 818 (1984), St. Louis, MO, April 1984.
19. F. W. Kleinhans, S. T. Barefoot and D. A. Hawley, Localization of Hydroxyl Radical Productions in Stimulated PMN using EPR, Biophysical J. 45, 242a (1984), San Antonio, TX, Feb. 1984.
18. S. T. Barefoot, D. A. Hawley and F. W. Kleinhans, EPR Assay of the Metabolic Burst of Polymorphonuclear Leukocytes, Bull. Amer. Phy. Soc. 28, 908 (1983), Bowling Green, OH, Oct. 1982.
17. F. W. Kleinhans, D. A. Hawley, J. L. Biesecker and M. R. Goodman, Detection of Immune Complexes in Serum of Patients with Systemic Lupus Erythematosus by Spin Resonance Spectroscopy of Complement Stimulated Leukocytes, Clin. Res. 30, 750A (1982).
16. D. A. Hawley, F. W. Kleinhans, J. L. Biesecker and M. R. Goodman, Detection of Immune Complexes Based on Spin-Trapping of Free Radicals Produced by Leukocytes, Annals Int. Med., 1982, Amer. Soc. Clin. Pathol. Meeting, Miami, FL, Oct. 1982.
15. F. W. Kleinhans, D. A. Hawley, B. D. Davies, J. L. Biesecker, M. R. Goodman, EPR Assay of Serum from Systemic Lupus Erythematosus Patients, Bull. Amer. Phy. Soc. 26, 1223 (1981), New Orleans, LA, Nov. 1981.
14. F. W. Kleinhans, S. C. Kline and W. M. Dugan, Comparison of EPR and AA Serum Copper Levels in Normals and Cancers, Amer. Phys. Soc., Granville OH, May 1981.
13. F. W. Kleinhans, S. C. Kline and W. M. Dugan, EPR and Total Serum Copper Measurements in Human Normals and Cancer Patients, Federation Proc. 39, 1769 (1980), New Orleans, LA, June 1980.
12. F. W. Kleinhans, S. C. Kline and W. M. Dugan, EPR and AA Serum Copper Measurements in Human Normals and Cancer Patients, Proc. Amer. Soc. Clin. Oncology 21, 325 (1980).
11. M. D. Kemple, F. W. Kleinhans, N. D. Lees and M. Bard, EPR Study of Membranes of Yeast Sterol Mutants, Bull. Amer. Phy. Soc. 24, 346 (1979).
10. W. S. Collier, D. Hood, J. Seubert, M. D.. Kemple, F. W. Kleinhans, Photographic and Visual Observations of the Feb. 26, 1979 Total Solar Eclipse, Bull. Amer. Phys. Soc. 24, 800 (1979), Youngstown, Ohio, Oct. 1979.
9. F. W. Kleinhans, N. D. Lees, M. Bard, R. A. Haak, ESR Determination of Membrane Order in Yeast Sterol Mutants, Biophys. J. 21, 36a (1978), Washington, D.C., March 1978.
8. W. D. Sawyer, W. J. Newhall, R. S. Rosenthal, F. W. Kleinhans and R. A. Haak, Spin Label ESR Studies of Membranes of Drug Susceptible and Resistant Gonococci, in P. Matagkosombut, ed., Abstracts of Fifth International Congress on Global Impacts of Applied Microbiology, Mahidol, Bangkok (1978).
7. F. W. Kleinhans, M. Bard, N. D. Lees and R. A. Haak, Increased Permeability of Yeast Membrane Mutants to Ni++ as Detected by ESR, Bull. Amer. Phys. Soc. 22, 277 (1977), San Diego, CA, March (1977).
6. R. A. Haak, F. W. Kleinhans, W. J. Newhall and S. Ochs, A Step Increase in Nerve Microviscosity at the Temperature Causing Cold Block of Fast Axoplasmic Transport, Biophys. J. 17, 275a (1977).
5. W. J. Newhall, F. W. Kleinhans, R. S. Rosenthal, M. K. Stine, W. D. Sawyer and R. A. Haak, Characterization of Neisseria gonorrhoeae by Spin Label ESR, Biophys. J. 17, 71a (1977).
4. W. J. Newhall, F. W. Kleinhans, S. Ochs and R. A. Haak, Temperature Dependence of Axoplasmic Microviscosity in Cat Sciatic Nerve, Bull. Amer. Phys. Soc., Oct. (1975).
3. R. A. Haak, F. W. Kleinhans and S. Ochs, ESR Spin Label Measure of Viscosity of Mammalian Nerve Axoplasm, Biophys. J. 15, 40a (1975).
2. F. W. Kleinhans and P. E. Wigen, Electron Paramagnetic Resonance in Mg-Mn, 17th Conference on Magnetism and Magnetic Materials, Chicago, IL, Fall (1971).
1. P. E. Wigen and F. W. Kleinhans, Logarithmic g Shift of Cobalt Paramagnetic Resonance in Molybdenum and Tungsten Host, Bull. Amer. Phys. Soc. 14, 313 (1969).
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